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青霉素G(BP)ELISA快速檢測試劑盒

一、原理

本試劑盒采用間接競爭ELISA方法，在微孔條上預包被偶聯抗原，樣本中的殘留物青霉素將和微孔條上預包被的偶聯抗原競爭抗青霉素抗體，加入酶標記物后，用TMB底物顯色，樣本吸光值與其所含殘留物青霉素的含量成負相關，與標準曲線比較即可得出相應殘留物青霉素的含量。

二、試劑盒特性

u  試劑盒靈敏度：   0.1ppb

u  孵育溫度：            25℃

u  孵育時間：           30min～30min～15min

u  批內、批間差： 不超過10%

u  樣本檢測下限

組織  ················································  2ppb

u  交叉反應率

青霉素·   ········································  100%

氨芐青霉素········································  0.8%

鄰氯青霉素 ·······································  0.2%

雙氯青霉素 ·······································  0.1%

阿莫西林  ·········································  0.1%

頭孢塞呋  ·········································  0.1%

u  樣本回收率

組  織 ·········································  60%-120%

三、試劑盒組成

四、樣品前處理步驟及檢測分析步驟

  詳見產品說明書。

五、儲藏條件和保質期

1、 儲藏條件：試劑盒于2-8℃保存，不要冷凍。

2、 保 質 期：該產品有效期為1年，生產日期見包裝盒。

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